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NOVA 制冷光谱仪 | 构建 532nm 显微荧光系统

新葡萄奔驰AMG用kun拉曼光谱仪、532nm 拉曼激光器和显微镜帮助客户一起实现一套低成本的显微拉曼系统。

显微光谱系统
显微光谱系统，即微区光谱系统或显微分光光度计，在显微镜的基础之上增加了光谱分析的功能。能够实现微米级样品的反射光谱、透射光谱、荧光光谱、拉曼光谱等光谱分析。显微荧光光谱测量系统是一套能够实现微米级物体荧光光谱采集的仪器，它不仅保持了显微镜对微小区域实时成像的特点，更具备了采集该区域物体 350 ~ 2500nm 波段内光谱的能力。

图1，100X 物镜下的定位光圈及激光斑

  背景
      特种荧光光纤在 532nm 激光激发下会在 600nm 附近产生一个较为宽的荧光峰，增大 532nm 激光的光强，会发现荧光峰峰位和峰型的变化。
      本案例运用了基于复享光谱仪以及定制的两种显微光路切换器所搭建的显微荧光测量系统，对特种荧光光纤进行了 1μm 区域内的荧光激发以及荧光光谱测量。

实验搭建

基于光谱仪 PG2000-Pro （ 200 ~ 1100nm）、M 型光纤（FIB-M-600-NIR）；双光路切换器（DPS）、C 口光路切换器（CMS）、532nm 激光器、光纤可调衰减器（FVA-LVF）等进行外部光路搭建实现显微荧光光谱测量系统，分别在低激光功率和高激光功率激发两个模式下对特种荧光光纤样品微米量级测量区域进行定点荧光激发测量，获得其荧光光谱。

图2，特种荧光光纤在不同功率激光激发下的显微荧光光谱

对测得光谱进行数据处理后发现，特种荧光光纤样品，在功率较低的 532nm 激光功率下激发出一个 600nm 左右的宽荧光峰。当激光功率增大时，荧光峰的峰型变窄，且峰位具有一定的蓝移。与预期测量结果一致。

结论
通过显微荧光光谱测量系统可以精确测量微米级区域内样品的荧光光谱，并且可以实时观测光谱采集的样品区域轮廓及样貌。

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制冷型光谱仪

显微镜光谱拓展口

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上一篇： R1 在光辐射调控中的应用

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NOVA 制冷光谱仪 | 构建 532nm 显微荧光系统

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